Biotin-11-UTP is a chemically modified analog of uridine triphosphate (UTP) designed for incorporation into RNA molecules during in vitro transcription using various RNA polymerases. This biotin-labeled nucleotide enables the generation of RNA probes that can be detected with high sensitivity in a wide range of molecular biology applications, including in situ hybridization, gene expression microarrays, and fluorescence in situ hybridization (FISH).

The “11” in its name refers to the length of the carbon linker—comprising 11 atoms—that connects the biotin moiety to the UTP base. This extended spacer arm helps minimize steric hindrance, improving accessibility for streptavidin conjugates used in detection. The biotin tag also allows for flexible visualization strategies, including direct fluorescence or enzymatic amplification, making Biotin-11-UTP a valuable tool for researchers working with low-abundance RNA targets or limited sample quantities.