Sensitivity and specificity：This anti-Npas4 antibody has minimal non-specific background binding as assessed by immunocytochemistry on primary neurons, immunohistochemistry on brain slices and be Western blots. Further, immunoreactivity is completely lost in Npas4 KO mice. Immunoreactivity in mouse and rat has been demonstrated. Not tested in other species.
Antibody solution and concentration：Each vial contains 100 µl of a recombinant rabbit monoclonal anti-Npas4 antibody solution diluted to 1 mg/ml in filter sterile PBS.
Applications and Dilution：We would recommend a 1:500 – 1:2000 dilution as a starting point for staining cultured neurons, immunohistochemistry or Westerns. But of course, optimal dilution factors will vary depending upon the specifics of your procedure.
Storage：The antibody is shipped on ice packs. Please keep at +4°C temporarily and upon first opportunity aliquot and freeze at -20°C for long-term storage. After thawing aliquots for use, each aliquot should be kept at +4°C for up to 1 month.

1.What makes Npas4 a superior marker for neuronal activity?
Npas4 offers unmatched specificity for neuron-specific activity with minimal non-specific background binding. This makes it an ideal marker for accurately identifying neuronal activity, providing clearer insights than traditional IEGs.
2.Why stain for Npas4 instead of cFos?
Unlike cFos, Npas4 is a more pure marker of neuronal activity. That said, cFos is widely used and very informative. We do not recommend using Npas4 instead of cFos, but rather recommend using it along with with cFos. Excellent compatible cFos antibodies are available with Synaptic Systems and are sold with our Npas4 antibody in Translucence Biosystem’s Neuronal Activity Tissue Clearing kits
3.What dilution should I start with for my application?
For most applications, including immunocytochemistry, immunohistochemistry, and Western blotting, a starting dilution of 1:500 to 1:2000 is recommended. However, the optimal dilution should be determined experimentally, depending on the specifics of your application and research needs.
4.What dilution factors are recommended for different applications?
Start with a dilution of 1:500 – 1:2000 for staining cultured neurons, immunohistochemistry, or Western blotting, adjusting as needed based on experimental results.
5.Is the Npas4 antibody reactive with species other than mice and rats?
The Npas4 antibody has been validated for use and shown immunoreactivity in mouse, rat and human tissues. It has not been extensively tested in other species. Researchers aiming to use the antibody with different species should conduct preliminary validation experiments to assess its suitability.
6.How should I store the Npas4 antibody for optimal longevity?
Store the antibody temporarily at +4°C upon arrival. For long-term storage, aliquot and freeze at -20°C. Thawed aliquots can be kept at +4°C for up to one month, ensuring the antibody's performance.
7.How do you ensure the antibody remains intact during shipping?
The Npas4 antibody is shipped with ice packs to maintain a stable, low temperature throughout transit. This careful handling is designed to preserve the antibody's quality and performance until it reaches your laboratory. The antibody is very stable and remains functional after prolonged periods at room temperature.